The 57-second video recorded at the Texas Health Presbyterian Hospital Dallas and released on YouTube today shows Ms Pham having a brief conversation with her treating physician.
Ms Pham has been transferred from Texas to the National Institutes of Health in Maryland, just outside Washington.
"Come to Maryland, everybody," she jokes.
The video, which was shot by the doctor, also shows another health care worker in attendance, wearing a full mask and other protective gear.
"I love you guys," an #emotional Ms Pham says, as she wipes away tears.
According to the hospital, Ms Pham asked for the video to be posted.
Ms Pham wore a white protective suit as she stepped off the plane in Maryland. She was later sped by ambulance to the NIH several miles away.
Anthony Fauci, head of the National Institute for Allergy and Infectious Diseases, told a congressional hearing earlier Thursday that Ms Pham would receive superlative care at NIH, one of just a few facilities equipped to provide comprehensive medical care for patients suffering from Ebola and other infectious agents.
"We will be supplying her with state-of-the-art care in our high-level containment facilities," Mr Fauci told lawmakers.
In a statement, the NIH said that the facility where Ms Pham will be treated is "staffed by infectious diseases and critical care specialists... trained in strict infection control practices optimized to prevent spread of potentially transmissible agents such as Ebola."
Ms Pham was closely involved in the care of a Liberian man, Thomas Eric Duncan, who was the first Ebola case diagnosed in the US.
He died of Ebola on October 8 at the Texas hospital.